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1.Screening
1.Inclusion Criteria

Age: > 18 years

Yes

Decompensated Liver Cirrhosis

Yes

Etiology: alcoholic or posthepatitic cirrhosis

Yes

Endoscopy was tolerable after anesthesia evaluation

Yes

2.Exclusion Criteria

Pregnant or lactating women

No

Patients with severe anemia and coagulation dysfunction that could not be improved after treatmen

No

Failure of hemostasis treatment for gastrointestinal bleeding in the past month

No

Presence of spontaneous peritonitis, hepatic encephalopathy, hepatorenal syndrome and acute infection

No

Presence of coexisting severe heart, lung, kidney, blood system or other diseases, history of mental illness

No

Malignant tumor of the liver or other organs

No

2.Baseline
1.General information

Gender

Male

Age

30 years

2.Clinical Symptoms

Clinical Symptoms

呕血、黑便

3.Etiology

Posthepatitic cirrhosis

Yes

Alcoholic liver cirrhosis

No

4.Blood Routine Tests

Hemoglobin

63 g/L

Red blood cell

2.15 *10^12/L

Platelet

61 *10^9/L

White blood cell

2.8 *10^9/L

5.Serum Liver Function Tests

Alanine aminotransferase

26 U/L

Aspartate aminotransferase

43 U/L

Total bilirubin

28.3 µmol/L

Albumin

26.2 g/L

6.Coagulation Function Tests

Prothrombin time

18 sec

Activated partial thromboplastin time

48.6 sec

Thrombin time

20 sec

Fibrinogen

1.48 g/L

7.Liver Fibrotest

Hyaluronic acid

124.35 ng/ml

Laminin

41.15 ng/ml

Collagen Ⅳ

77.16 ng/ml

Procollagen Ⅲ

74.2 ng/ml

8.Ascites

Ascites

101 mm

9.Portal Vein Diameter

Portal vein diameter

11 mm

10.Neoplastic Lesions

Neoplastic lesions

No

11.Portal Vein Thrombosis

Portal vein thrombosis

No

12.Liver Stiffness

Liver stiffness

37.9 KPA

13.Fat Attenuation Parameters

Fat attenuation parameters

235 db/m

14.Child-Pugh Scores

Child-Pugh scores

10

3.In the process of the treatment
1.Treatment Protocol

Treatment protocol

①骨髓采集共约30ml;②超声内镜引导下经胃经肝门静脉穿刺;③经门静脉输注自体骨髓。

2.Treatment Completed

Treatment completed

Yes

4.Adverse Events
1.Hemorrhage

Hemorrhage

No

2.Hematoma

Hematoma

No

3.Fever

Fever

No

4.Infection

Infection

No

5.Pain at the puncture site

Pain at the puncture site

No

6.Acute liver failure

Acute liver failure

No

7.Hepatic encephalopathy

Hepatic encephalopathy

No

5.Follow-up 1
1.Clinical Symptoms

Clinical symptoms

无呕血、黑便

2.Blood Routine Tests

Hemoglobin

58 g/L

Red blood cell

2.5 *10^12/L

Platelet

59 *10^9/L

White blood cell

2.57 *10^9/L

3.Serum Liver Function Tests

Alanine aminotransferase

24 U/L

Aspartate aminotransferase

30 U/L

Total bilirubin

19.3 µmol/L

Albumin

34.3 g/L

4.Coagulation Function Tests

Prothrombin time

15.6 sec

Activated partial thromboplastin time

33.1 sec

Thrombin time

16.5 sec

Fibrinogen

1.39 g/L

5.Liver Fibrotest

Hyaluronic acid

90.62 ng/ml

Laminin

56.61 ng/ml

Collagen Ⅳ

68.97 ng/ml

Procollagen Ⅲ

65.78 ng/ml

6.Ascites

Ascites

87 mm

7.Portal Vein Diameter

Portal vein diameter

14 mm

8.Neoplastic Lesions

Neoplastic lesions

No

9.Portal Vein Thrombosis

Portal vein thrombosis

No

10.Liver Stiffness

Liver stiffness

12.4 KPA

11.Fat Attenuation Parameters

Fat attenuation parameters

217 db/m

12.Child-Pugh Scores

Child-Pugh scores

8

6.Follow-up 2
1.Clinical Symptoms

Clinical symptoms

无呕血、黑便

2.Blood Routine Tests

Hemoglobin

88 g/L

Red blood cell

4.62 *10^12/L

Platelet

44 *10^9/L

White blood cell

2.14 *10^9/L

3.Serum Liver Function Tests

Alanine aminotransferase

45 U/L

Aspartate aminotransferase

54 U/L

Total bilirubin

20.1 µmol/L

Albumin

34.5 g/L

4.Coagulation Function Tests

Prothrombin time

15.6 sec

Activated partial thromboplastin time

41.8 sec

Thrombin time

19.5 sec

Fibrinogen

1.5 g/L

5.Liver Fibrotest

Hyaluronic acid

94.56 ng/ml

Laminin

45.31 ng/ml

Collagen Ⅳ

66.29 ng/ml

Procollagen Ⅲ

65.42 ng/ml

6.Ascites

Ascites

40 mm

7.Portal Vein Diameter

Portal vein diameter

13 mm

8.Neoplastic Lesions

Neoplastic lesions

No

9.Portal Vein Thrombosis

Portal vein thrombosis

No

10.Liver Stiffness

Liver stiffness

21.5 KPA

11.Fat Attenuation Parameters

Fat attenuation parameters

220 db/m

12.Child-Pugh Scores

Child-Pugh scores

8

7.Follow-up 3
1.Clinical Symptoms

Clinical symptoms

无呕血、黑便

2.Blood Routine Tests

Hemoglobin

97 g/L

Red blood cell

4.78 *10^12/L

Platelet

49 *10^9/L

White blood cell

2.58 *10^9/L

3.Serum Liver Function Tests

Alanine aminotransferase

45 U/L

Aspartate aminotransferase

52 U/L

Total bilirubin

24.8 µmol/L

Albumin

36.3 g/L

4.Coagulation Function Tests

Prothrombin time

16.3 sec

Activated partial thromboplastin time

43.8 sec

Thrombin time

18.1 sec

Fibrinogen

1.75 g/L

5.Liver Fibrotest

Hyaluronic acid

160.3 ng/ml

Laminin

109.3 ng/ml

Collagen Ⅳ

46.56 ng/ml

Procollagen Ⅲ

48.12 ng/ml

6.Ascites

Ascites

0 mm

7.Portal Vein Diameter

Portal vein diameter

10 mm

8.Neoplastic Lesions

Neoplastic lesions

No

9.Portal Vein Thrombosis

Portal vein thrombosis

No

10.Liver Stiffness

Liver stiffness

20.3 KPA

11.Fat Attenuation Parameters

Fat attenuation parameters

240 db/m

12.Child-Pugh Scores

Child-Pugh scores

5

8.Follwo-up 4
1.Clinical Symptoms

Clinical symptoms

无呕血、黑便

2.Blood Routine Tests

Hemoglobin

94 g/L

Red blood cell

4.81 *10^12/L

Platelet

53 *10^9/L

White blood cell

2.85 *10^9/L

3.Serum Liver Function Tests

Alanine aminotransferase

43 U/L

Aspartate aminotransferase

50 U/L

Total bilirubin

28.9 µmol/L

Albumin

30.6 g/L

4.Coagulation Function Tests

Prothrombin time

19.2 sec

Activated partial thromboplastin time

46.6 sec

Thrombin time

20.7 sec

Fibrinogen

1.3 g/L

5.Liver Fibrotest

Hyaluronic acid

106.02 ng/ml

Laminin

27.9 ng/ml

Collagen Ⅳ

66.07 ng/ml

Procollagen Ⅲ

22.72 ng/ml

6.Ascites

Ascites

56 mm

7.Portal Vein Diameter

Portal vein diameter

11 mm

8.Neoplastic Lesions

Neoplastic lesions

No

9.Portal Vein Thrombosis

Portal vein thrombosis

No

10.Liver Stiffness

Liver stiffness

44.3 KPA

11.Fat Attenuation Parameters

Fat attenuation parameters

240 db/m

12.Child-Pugh Scores

Child-Pugh scores

9

9.Management system of participants
10.Speciman management system
11.Data management system
12.ADR/ADE management system
13.Instruments management system
14.SOPs
1.Treatment SOP
2.Nursing SOP
15.Research team
1.Institutions
2.Researchers