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1.Screening
1.Inclusion Criteria

Age: > 18 years

Yes

Decompensated Liver Cirrhosis

Yes

Etiology: alcoholic or posthepatitic cirrhosis

Yes

Endoscopy was tolerable after anesthesia evaluation

Yes

2.Exclusion Criteria

Pregnant or lactating women

No

Patients with severe anemia and coagulation dysfunction that could not be improved after treatmen

No

Failure of hemostasis treatment for gastrointestinal bleeding in the past month

No

Presence of spontaneous peritonitis, hepatic encephalopathy, hepatorenal syndrome and acute infection

No

Presence of coexisting severe heart, lung, kidney, blood system or other diseases, history of mental illness

No

Malignant tumor of the liver or other organs

No

2.Baseline
1.General information

Gender

Female

Age

71 years

2.Clinical Symptoms

Clinical Symptoms

腹胀

3.Etiology

Posthepatitic cirrhosis

Yes

Alcoholic liver cirrhosis

No

4.Blood Routine Tests

Hemoglobin

107 g/L

Red blood cell

3.05 *10^12/L

Platelet

92 *10^9/L

White blood cell

3.34 *10^9/L

5.Serum Liver Function Tests

Alanine aminotransferase

46 U/L

Aspartate aminotransferase

82 U/L

Total bilirubin

18.3 µmol/L

Albumin

23.5 g/L

6.Coagulation Function Tests

Prothrombin time

13.3 sec

Activated partial thromboplastin time

37.7 sec

Thrombin time

19.5 sec

Fibrinogen

1.6 g/L

7.Liver Fibrotest

Hyaluronic acid

674.7 ng/ml

Laminin

33.83 ng/ml

Collagen Ⅳ

122.5 ng/ml

Procollagen Ⅲ

125.5 ng/ml

8.Ascites

Ascites

80 mm

9.Portal Vein Diameter

Portal vein diameter

10 mm

10.Neoplastic Lesions

Neoplastic lesions

No

11.Portal Vein Thrombosis

Portal vein thrombosis

No

12.Liver Stiffness

Liver stiffness

29.8 KPA

13.Fat Attenuation Parameters

Fat attenuation parameters

220 db/m

14.Child-Pugh Scores

Child-Pugh scores

9

3.In the process of the treatment
1.Treatment Protocol

Treatment protocol

①骨髓采集共约30ml;②超声内镜引导下经胃经肝门静脉穿刺;③经门静脉输注自体骨髓。

2.Treatment Completed

Treatment completed

Yes

4.Adverse Events
1.Hemorrhage

Hemorrhage

No

2.Hematoma

Hematoma

No

3.Fever

Fever

No

4.Infection

Infection

No

5.Pain at the puncture site

Pain at the puncture site

No

6.Acute liver failure

Acute liver failure

No

7.Hepatic encephalopathy

Hepatic encephalopathy

No

5.Follow-up 1
1.Clinical Symptoms

Clinical symptoms

腹胀改善

2.Blood Routine Tests

Hemoglobin

102 g/L

Red blood cell

3.06 *10^12/L

Platelet

90 *10^9/L

White blood cell

3.5 *10^9/L

3.Serum Liver Function Tests

Alanine aminotransferase

35 U/L

Aspartate aminotransferase

71 U/L

Total bilirubin

14 µmol/L

Albumin

32 g/L

4.Coagulation Function Tests

Prothrombin time

13.5 sec

Activated partial thromboplastin time

41.5 sec

Thrombin time

20.4 sec

Fibrinogen

1.5 g/L

5.Liver Fibrotest

Hyaluronic acid

667.3 ng/ml

Laminin

38.4 ng/ml

Collagen Ⅳ

136.3 ng/ml

Procollagen Ⅲ

139.6 ng/ml

6.Ascites

Ascites

115 mm

7.Portal Vein Diameter

Portal vein diameter

12 mm

8.Neoplastic Lesions

Neoplastic lesions

No

9.Portal Vein Thrombosis

Portal vein thrombosis

No

10.Liver Stiffness

Liver stiffness

30.1 KPA

11.Fat Attenuation Parameters

Fat attenuation parameters

233 db/m

12.Child-Pugh Scores

Child-Pugh scores

8

6.Follow-up 2
1.Clinical Symptoms

Clinical symptoms

腹胀改善

2.Blood Routine Tests

Hemoglobin

110 g/L

Red blood cell

3.09 *10^12/L

Platelet

97 *10^9/L

White blood cell

3.5 *10^9/L

3.Serum Liver Function Tests

Alanine aminotransferase

32 U/L

Aspartate aminotransferase

45 U/L

Total bilirubin

10.7 µmol/L

Albumin

34 g/L

4.Coagulation Function Tests

Prothrombin time

13.7 sec

Activated partial thromboplastin time

37.5 sec

Thrombin time

19.9 sec

Fibrinogen

1.6 g/L

5.Liver Fibrotest

Hyaluronic acid

455.62 ng/ml

Laminin

35.5 ng/ml

Collagen Ⅳ

125.67 ng/ml

Procollagen Ⅲ

132.58 ng/ml

6.Ascites

Ascites

76 mm

7.Portal Vein Diameter

Portal vein diameter

10 mm

8.Neoplastic Lesions

Neoplastic lesions

No

9.Portal Vein Thrombosis

Portal vein thrombosis

No

10.Liver Stiffness

Liver stiffness

24.5 KPA

11.Fat Attenuation Parameters

Fat attenuation parameters

220 db/m

12.Child-Pugh Scores

Child-Pugh scores

8

7.Follow-up 3
1.Clinical Symptoms

Clinical symptoms

腹胀改善

2.Blood Routine Tests

Hemoglobin

120 g/L

Red blood cell

3.56 *10^12/L

Platelet

122 *10^9/L

White blood cell

3.9 *10^9/L

3.Serum Liver Function Tests

Alanine aminotransferase

27 U/L

Aspartate aminotransferase

34 U/L

Total bilirubin

9 µmol/L

Albumin

49 g/L

4.Coagulation Function Tests

Prothrombin time

13.9 sec

Activated partial thromboplastin time

34.2 sec

Thrombin time

16.2 sec

Fibrinogen

2.85 g/L

5.Liver Fibrotest

Hyaluronic acid

396.8 ng/ml

Laminin

26.87 ng/ml

Collagen Ⅳ

87.45 ng/ml

Procollagen Ⅲ

91.05 ng/ml

6.Ascites

Ascites

38 mm

7.Portal Vein Diameter

Portal vein diameter

10 mm

8.Neoplastic Lesions

Neoplastic lesions

No

9.Portal Vein Thrombosis

Portal vein thrombosis

No

10.Liver Stiffness

Liver stiffness

19 KPA

11.Fat Attenuation Parameters

Fat attenuation parameters

210 db/m

12.Child-Pugh Scores

Child-Pugh scores

7

8.Follwo-up 4
1.Clinical Symptoms

Clinical symptoms

腹胀改善

2.Blood Routine Tests

Hemoglobin

112 g/L

Red blood cell

3.8 *10^12/L

Platelet

103 *10^9/L

White blood cell

3.86 *10^9/L

3.Serum Liver Function Tests

Alanine aminotransferase

102 U/L

Aspartate aminotransferase

224 U/L

Total bilirubin

22 µmol/L

Albumin

42 g/L

4.Coagulation Function Tests

Prothrombin time

12.3 sec

Activated partial thromboplastin time

37.8 sec

Thrombin time

14.7 sec

Fibrinogen

2.1 g/L

5.Liver Fibrotest

Hyaluronic acid

403.8 ng/ml

Laminin

24.9 ng/ml

Collagen Ⅳ

77.2 ng/ml

Procollagen Ⅲ

74.1 ng/ml

6.Ascites

Ascites

22 mm

7.Portal Vein Diameter

Portal vein diameter

10 mm

8.Neoplastic Lesions

Neoplastic lesions

No

9.Portal Vein Thrombosis

Portal vein thrombosis

No

10.Liver Stiffness

Liver stiffness

25.3 KPA

11.Fat Attenuation Parameters

Fat attenuation parameters

215 db/m

12.Child-Pugh Scores

Child-Pugh scores

6

9.Management system of participants
10.Speciman management system
11.Data management system
12.ADR/ADE management system
13.Instruments management system
14.SOPs
1.Treatment SOP
2.Nursing SOP
15.Research team
1.Institutions
2.Researchers