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1.Screening
1.Inclusion Criteria

Age: > 18 years

Yes

Decompensated Liver Cirrhosis

Yes

Etiology: alcoholic or posthepatitic cirrhosis

Yes

Endoscopy was tolerable after anesthesia evaluation

Yes

2.Exclusion Criteria

Pregnant or lactating women

No

Patients with severe anemia and coagulation dysfunction that could not be improved after treatmen

No

Failure of hemostasis treatment for gastrointestinal bleeding in the past month

No

Presence of spontaneous peritonitis, hepatic encephalopathy, hepatorenal syndrome and acute infection

No

Presence of coexisting severe heart, lung, kidney, blood system or other diseases, history of mental illness

No

Malignant tumor of the liver or other organs

No

2.Baseline
1.General information

Gender

Male

Age

44 years

2.Clinical Symptoms

Clinical Symptoms

腹胀

3.Etiology

Posthepatitic cirrhosis

Yes

Alcoholic liver cirrhosis

No

4.Blood Routine Tests

Hemoglobin

136 g/L

Red blood cell

3.91 *10^12/L

Platelet

28 *10^9/L

White blood cell

2.18 *10^9/L

5.Serum Liver Function Tests

Alanine aminotransferase

41 U/L

Aspartate aminotransferase

52 U/L

Total bilirubin

38 µmol/L

Albumin

27.2 g/L

6.Coagulation Function Tests

Prothrombin time

16 sec

Activated partial thromboplastin time

40.3 sec

Thrombin time

23.4 sec

Fibrinogen

0.99 g/L

7.Liver Fibrotest

Hyaluronic acid

405.76 ng/ml

Laminin

204.4 ng/ml

Collagen Ⅳ

67.56 ng/ml

Procollagen Ⅲ

81.95 ng/ml

8.Ascites

Ascites

14 mm

9.Portal Vein Diameter

Portal vein diameter

11 mm

10.Neoplastic Lesions

Neoplastic lesions

No

11.Portal Vein Thrombosis

Portal vein thrombosis

No

12.Liver Stiffness

Liver stiffness

24.8 KPA

13.Fat Attenuation Parameters

Fat attenuation parameters

283 db/m

14.Child-Pugh Scores

Child-Pugh scores

9

3.In the process of the treatment
1.Treatment Protocol

Treatment protocol

①骨髓采集共约30ml;②超声内镜引导下经胃经肝门静脉穿刺;③经门静脉输注自体骨髓。

2.Treatment Completed

Treatment completed

Yes

4.Adverse Events
1.Hemorrhage

Hemorrhage

No

2.Hematoma

Hematoma

No

3.Fever

Fever

No

4.Infection

Infection

No

5.Pain at the puncture site

Pain at the puncture site

No

6.Acute liver failure

Acute liver failure

No

7.Hepatic encephalopathy

Hepatic encephalopathy

No

5.Follow-up 1
1.Clinical Symptoms

Clinical symptoms

腹胀改善

2.Blood Routine Tests

Hemoglobin

149 g/L

Red blood cell

4.38 *10^12/L

Platelet

29 *10^9/L

White blood cell

3.01 *10^9/L

3.Serum Liver Function Tests

Alanine aminotransferase

49 U/L

Aspartate aminotransferase

64 U/L

Total bilirubin

44.2 µmol/L

Albumin

31 g/L

4.Coagulation Function Tests

Prothrombin time

14.6 sec

Activated partial thromboplastin time

34.6 sec

Thrombin time

23.7 sec

Fibrinogen

1.03 g/L

5.Liver Fibrotest

Hyaluronic acid

342.88 ng/ml

Laminin

328.9 ng/ml

Collagen Ⅳ

59.99 ng/ml

Procollagen Ⅲ

65.36 ng/ml

6.Ascites

Ascites

17 mm

7.Portal Vein Diameter

Portal vein diameter

12 mm

8.Neoplastic Lesions

Neoplastic lesions

No

9.Portal Vein Thrombosis

Portal vein thrombosis

No

10.Liver Stiffness

Liver stiffness

19.4 KPA

11.Fat Attenuation Parameters

Fat attenuation parameters

320 db/m

12.Child-Pugh Scores

Child-Pugh scores

8

6.Follow-up 2
1.Clinical Symptoms

Clinical symptoms

腹胀改善

2.Blood Routine Tests

Hemoglobin

138 g/L

Red blood cell

4.26 *10^12/L

Platelet

29 *10^9/L

White blood cell

2.94 *10^9/L

3.Serum Liver Function Tests

Alanine aminotransferase

38 U/L

Aspartate aminotransferase

42 U/L

Total bilirubin

47.5 µmol/L

Albumin

28.5 g/L

4.Coagulation Function Tests

Prothrombin time

15 sec

Activated partial thromboplastin time

36.2 sec

Thrombin time

21.9 sec

Fibrinogen

1.04 g/L

5.Liver Fibrotest

Hyaluronic acid

355.81 ng/ml

Laminin

287.6 ng/ml

Collagen Ⅳ

60.46 ng/ml

Procollagen Ⅲ

66.23 ng/ml

6.Ascites

Ascites

70 mm

7.Portal Vein Diameter

Portal vein diameter

10 mm

8.Neoplastic Lesions

Neoplastic lesions

No

9.Portal Vein Thrombosis

Portal vein thrombosis

No

10.Liver Stiffness

Liver stiffness

18.3 KPA

11.Fat Attenuation Parameters

Fat attenuation parameters

240 db/m

12.Child-Pugh Scores

Child-Pugh scores

9

7.Follow-up 3
1.Clinical Symptoms

Clinical symptoms

腹胀有所改善

2.Blood Routine Tests

Hemoglobin

130 g/L

Red blood cell

3.75 *10^12/L

Platelet

25 *10^9/L

White blood cell

2.2 *10^9/L

3.Serum Liver Function Tests

Alanine aminotransferase

27 U/L

Aspartate aminotransferase

38 U/L

Total bilirubin

44.2 µmol/L

Albumin

25.2 g/L

4.Coagulation Function Tests

Prothrombin time

15.4 sec

Activated partial thromboplastin time

37.6 sec

Thrombin time

24.6 sec

Fibrinogen

1.13 g/L

5.Liver Fibrotest

Hyaluronic acid

305.53 ng/ml

Laminin

283.7 ng/ml

Collagen Ⅳ

78.51 ng/ml

Procollagen Ⅲ

84.2 ng/ml

6.Ascites

Ascites

77 mm

7.Portal Vein Diameter

Portal vein diameter

10 mm

8.Neoplastic Lesions

Neoplastic lesions

No

9.Portal Vein Thrombosis

Portal vein thrombosis

No

10.Liver Stiffness

Liver stiffness

18.9 KPA

11.Fat Attenuation Parameters

Fat attenuation parameters

275 db/m

12.Child-Pugh Scores

Child-Pugh scores

10

8.Follwo-up 4
1.Clinical Symptoms

Clinical symptoms

腹胀较前稍改善

2.Blood Routine Tests

Hemoglobin

117 g/L

Red blood cell

3.43 *10^12/L

Platelet

22 *10^9/L

White blood cell

1.71 *10^9/L

3.Serum Liver Function Tests

Alanine aminotransferase

31.75 U/L

Aspartate aminotransferase

36.08 U/L

Total bilirubin

39.19 µmol/L

Albumin

23.8 g/L

4.Coagulation Function Tests

Prothrombin time

15.4 sec

Activated partial thromboplastin time

43.7 sec

Thrombin time

23.9 sec

Fibrinogen

0.83 g/L

5.Liver Fibrotest

Hyaluronic acid

311.6 ng/ml

Laminin

228.4 ng/ml

Collagen Ⅳ

43.1 ng/ml

Procollagen Ⅲ

64.9 ng/ml

6.Ascites

Ascites

78 mm

7.Portal Vein Diameter

Portal vein diameter

13 mm

8.Neoplastic Lesions

Neoplastic lesions

No

9.Portal Vein Thrombosis

Portal vein thrombosis

No

10.Liver Stiffness

Liver stiffness

15.2 KPA

11.Fat Attenuation Parameters

Fat attenuation parameters

264 db/m

12.Child-Pugh Scores

Child-Pugh scores

8

9.Management system of participants
10.Speciman management system
11.Data management system
12.ADR/ADE management system
13.Instruments management system
14.SOPs
1.Treatment SOP
2.Nursing SOP
15.Research team
1.Institutions
2.Researchers