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1.Screening
1.Inclusion Criteria

Age: > 18 years

Yes

Decompensated Liver Cirrhosis

Yes

Etiology: alcoholic or posthepatitic cirrhosis

Yes

Endoscopy was tolerable after anesthesia evaluation

Yes

2.Exclusion Criteria

Pregnant or lactating women

No

Patients with severe anemia and coagulation dysfunction that could not be improved after treatmen

No

Failure of hemostasis treatment for gastrointestinal bleeding in the past month

No

Presence of spontaneous peritonitis, hepatic encephalopathy, hepatorenal syndrome and acute infection

No

Presence of coexisting severe heart, lung, kidney, blood system or other diseases, history of mental illness

No

Malignant tumor of the liver or other organs

No

2.Baseline
1.General information

Gender

Male

Age

57 years

2.Clinical Symptoms

Clinical Symptoms

腹胀、双下肢水肿

3.Etiology

Posthepatitic cirrhosis

Yes

Alcoholic liver cirrhosis

No

4.Blood Routine Tests

Hemoglobin

81 g/L

Red blood cell

2.74 *10^12/L

Platelet

78 *10^9/L

White blood cell

2.81 *10^9/L

5.Serum Liver Function Tests

Alanine aminotransferase

29 U/L

Aspartate aminotransferase

52 U/L

Total bilirubin

10.9 µmol/L

Albumin

25 g/L

6.Coagulation Function Tests

Prothrombin time

16 sec

Activated partial thromboplastin time

40.5 sec

Thrombin time

20.6 sec

Fibrinogen

1.64 g/L

7.Liver Fibrotest

Hyaluronic acid

297.91 ng/ml

Laminin

73.1 ng/ml

Collagen Ⅳ

57.27 ng/ml

Procollagen Ⅲ

58.48 ng/ml

8.Ascites

Ascites

86 mm

9.Portal Vein Diameter

Portal vein diameter

10 mm

10.Neoplastic Lesions

Neoplastic lesions

No

11.Portal Vein Thrombosis

Portal vein thrombosis

No

12.Liver Stiffness

Liver stiffness

18 KPA

13.Fat Attenuation Parameters

Fat attenuation parameters

246 db/m

14.Child-Pugh Scores

Child-Pugh scores

9

3.In the process of the treatment
1.Treatment Protocol

Treatment protocol

①骨髓采集共约30ml;②超声内镜引导下经胃经肝门静脉穿刺;③经门静脉输注自体骨髓。

2.Treatment Completed

Treatment completed

Yes

4.Adverse Events
1.Hemorrhage

Hemorrhage

No

2.Hematoma

Hematoma

No

3.Fever

Fever

No

4.Infection

Infection

No

5.Pain at the puncture site

Pain at the puncture site

No

6.Acute liver failure

Acute liver failure

No

7.Hepatic encephalopathy

Hepatic encephalopathy

No

5.Follow-up 1
1.Clinical Symptoms

Clinical symptoms

腹胀、水肿改善

2.Blood Routine Tests

Hemoglobin

101 g/L

Red blood cell

3.62 *10^12/L

Platelet

111 *10^9/L

White blood cell

3.9 *10^9/L

3.Serum Liver Function Tests

Alanine aminotransferase

25 U/L

Aspartate aminotransferase

45 U/L

Total bilirubin

18.3 µmol/L

Albumin

35.8 g/L

4.Coagulation Function Tests

Prothrombin time

13.2 sec

Activated partial thromboplastin time

34.3 sec

Thrombin time

20.9 sec

Fibrinogen

1.59 g/L

5.Liver Fibrotest

Hyaluronic acid

144.38 ng/ml

Laminin

61.31 ng/ml

Collagen Ⅳ

36.3 ng/ml

Procollagen Ⅲ

34.24 ng/ml

6.Ascites

Ascites

0 mm

7.Portal Vein Diameter

Portal vein diameter

14 mm

8.Neoplastic Lesions

Neoplastic lesions

No

9.Portal Vein Thrombosis

Portal vein thrombosis

No

10.Liver Stiffness

Liver stiffness

9.8 KPA

11.Fat Attenuation Parameters

Fat attenuation parameters

211 db/m

12.Child-Pugh Scores

Child-Pugh scores

5

6.Follow-up 2
1.Clinical Symptoms

Clinical symptoms

腹胀、水肿改善

2.Blood Routine Tests

Hemoglobin

108 g/L

Red blood cell

3.68 *10^12/L

Platelet

105 *10^9/L

White blood cell

3.87 *10^9/L

3.Serum Liver Function Tests

Alanine aminotransferase

22 U/L

Aspartate aminotransferase

48 U/L

Total bilirubin

13.6 µmol/L

Albumin

36.5 g/L

4.Coagulation Function Tests

Prothrombin time

12.8 sec

Activated partial thromboplastin time

30.9 sec

Thrombin time

21.1 sec

Fibrinogen

1.6 g/L

5.Liver Fibrotest

Hyaluronic acid

165.19 ng/ml

Laminin

46.62 ng/ml

Collagen Ⅳ

31.58 ng/ml

Procollagen Ⅲ

39.16 ng/ml

6.Ascites

Ascites

0 mm

7.Portal Vein Diameter

Portal vein diameter

13 mm

8.Neoplastic Lesions

Neoplastic lesions

No

9.Portal Vein Thrombosis

Portal vein thrombosis

No

10.Liver Stiffness

Liver stiffness

12.5 KPA

11.Fat Attenuation Parameters

Fat attenuation parameters

234 db/m

12.Child-Pugh Scores

Child-Pugh scores

5

7.Follow-up 3
1.Clinical Symptoms

Clinical symptoms

腹胀、水肿好转

2.Blood Routine Tests

Hemoglobin

86 g/L

Red blood cell

3.64 *10^12/L

Platelet

135 *10^9/L

White blood cell

3.67 *10^9/L

3.Serum Liver Function Tests

Alanine aminotransferase

18 U/L

Aspartate aminotransferase

44 U/L

Total bilirubin

15.4 µmol/L

Albumin

38.9 g/L

4.Coagulation Function Tests

Prothrombin time

12.1 sec

Activated partial thromboplastin time

31.9 sec

Thrombin time

24.1 sec

Fibrinogen

2.11 g/L

5.Liver Fibrotest

Hyaluronic acid

97.4 ng/ml

Laminin

38.43 ng/ml

Collagen Ⅳ

27.6 ng/ml

Procollagen Ⅲ

24.5 ng/ml

6.Ascites

Ascites

0 mm

7.Portal Vein Diameter

Portal vein diameter

10 mm

8.Neoplastic Lesions

Neoplastic lesions

No

9.Portal Vein Thrombosis

Portal vein thrombosis

No

10.Liver Stiffness

Liver stiffness

20.2 KPA

11.Fat Attenuation Parameters

Fat attenuation parameters

291 db/m

12.Child-Pugh Scores

Child-Pugh scores

5

8.Follwo-up 4
1.Clinical Symptoms

Clinical symptoms

腹胀、水肿好转

2.Blood Routine Tests

Hemoglobin

99 g/L

Red blood cell

4.01 *10^12/L

Platelet

120 *10^9/L

White blood cell

4.2 *10^9/L

3.Serum Liver Function Tests

Alanine aminotransferase

19 U/L

Aspartate aminotransferase

45 U/L

Total bilirubin

15.4 µmol/L

Albumin

43.2 g/L

4.Coagulation Function Tests

Prothrombin time

13.2 sec

Activated partial thromboplastin time

31.9 sec

Thrombin time

19.7 sec

Fibrinogen

2.38 g/L

5.Liver Fibrotest

Hyaluronic acid

161.56 ng/ml

Laminin

24.78 ng/ml

Collagen Ⅳ

24.03 ng/ml

Procollagen Ⅲ

18.81 ng/ml

6.Ascites

Ascites

0 mm

7.Portal Vein Diameter

Portal vein diameter

12 mm

8.Neoplastic Lesions

Neoplastic lesions

No

9.Portal Vein Thrombosis

Portal vein thrombosis

No

10.Liver Stiffness

Liver stiffness

17 KPA

11.Fat Attenuation Parameters

Fat attenuation parameters

245 db/m

12.Child-Pugh Scores

Child-Pugh scores

5

9.Management system of participants
10.Speciman management system
11.Data management system
12.ADR/ADE management system
13.Instruments management system
14.SOPs
1.Treatment SOP
2.Nursing SOP
15.Research team
1.Institutions
2.Researchers