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1.Screening
1.Inclusion Criteria

Age: > 18 years

Yes

Decompensated Liver Cirrhosis

Yes

Etiology: alcoholic or posthepatitic cirrhosis

Yes

Endoscopy was tolerable after anesthesia evaluation

Yes

2.Exclusion Criteria

Pregnant or lactating women

No

Patients with severe anemia and coagulation dysfunction that could not be improved after treatmen

No

Failure of hemostasis treatment for gastrointestinal bleeding in the past month

No

Presence of spontaneous peritonitis, hepatic encephalopathy, hepatorenal syndrome and acute infection

No

Presence of coexisting severe heart, lung, kidney, blood system or other diseases, history of mental illness

No

Malignant tumor of the liver or other organs

No

2.Baseline
1.General information

Gender

Male

Age

54 years

2.Clinical Symptoms

Clinical Symptoms

腹痛

3.Etiology

Posthepatitic cirrhosis

Yes

Alcoholic liver cirrhosis

No

4.Blood Routine Tests

Hemoglobin

115 g/L

Red blood cell

4.22 *10^12/L

Platelet

115 *10^9/L

White blood cell

2.07 *10^9/L

5.Serum Liver Function Tests

Alanine aminotransferase

27 U/L

Aspartate aminotransferase

36 U/L

Total bilirubin

14.9 µmol/L

Albumin

36 g/L

6.Coagulation Function Tests

Prothrombin time

16.8 sec

Activated partial thromboplastin time

31.7 sec

Thrombin time

17.9 sec

Fibrinogen

1.95 g/L

7.Liver Fibrotest

Hyaluronic acid

123.04 ng/ml

Laminin

25.86 ng/ml

Collagen Ⅳ

25.47 ng/ml

Procollagen Ⅲ

28.56 ng/ml

8.Ascites

Ascites

18 mm

9.Portal Vein Diameter

Portal vein diameter

16 mm

10.Neoplastic Lesions

Neoplastic lesions

No

11.Portal Vein Thrombosis

Portal vein thrombosis

No

12.Liver Stiffness

Liver stiffness

20.2 KPA

13.Fat Attenuation Parameters

Fat attenuation parameters

243 db/m

14.Child-Pugh Scores

Child-Pugh scores

6

3.In the process of the treatment
1.Treatment Protocol

Treatment protocol

①骨髓采集共约30ml;②超声内镜引导下经胃经肝门静脉穿刺;③经门静脉输注自体骨髓。

2.Treatment Completed

Treatment completed

Yes

4.Adverse Events
1.Hemorrhage

Hemorrhage

No

2.Hematoma

Hematoma

No

3.Fever

Fever

No

4.Infection

Infection

No

5.Pain at the puncture site

Pain at the puncture site

No

6.Acute liver failure

Acute liver failure

No

7.Hepatic encephalopathy

Hepatic encephalopathy

No

5.Follow-up 1
1.Clinical Symptoms

Clinical symptoms

腹痛好转

2.Blood Routine Tests

Hemoglobin

138 g/L

Red blood cell

5.22 *10^12/L

Platelet

138 *10^9/L

White blood cell

4.43 *10^9/L

3.Serum Liver Function Tests

Alanine aminotransferase

40 U/L

Aspartate aminotransferase

50 U/L

Total bilirubin

26.6 µmol/L

Albumin

45.7 g/L

4.Coagulation Function Tests

Prothrombin time

15.5 sec

Activated partial thromboplastin time

29.6 sec

Thrombin time

18 sec

Fibrinogen

2.1 g/L

5.Liver Fibrotest

Hyaluronic acid

186.9 ng/ml

Laminin

33.91 ng/ml

Collagen Ⅳ

21.68 ng/ml

Procollagen Ⅲ

22.96 ng/ml

6.Ascites

Ascites

16 mm

7.Portal Vein Diameter

Portal vein diameter

15 mm

8.Neoplastic Lesions

Neoplastic lesions

No

9.Portal Vein Thrombosis

Portal vein thrombosis

No

10.Liver Stiffness

Liver stiffness

55.6 KPA

11.Fat Attenuation Parameters

Fat attenuation parameters

236 db/m

12.Child-Pugh Scores

Child-Pugh scores

6

6.Follow-up 2
1.Clinical Symptoms

Clinical symptoms

腹痛好转

2.Blood Routine Tests

Hemoglobin

126 g/L

Red blood cell

4.61 *10^12/L

Platelet

33 *10^9/L

White blood cell

2.59 *10^9/L

3.Serum Liver Function Tests

Alanine aminotransferase

27 U/L

Aspartate aminotransferase

33 U/L

Total bilirubin

16.8 µmol/L

Albumin

39.7 g/L

4.Coagulation Function Tests

Prothrombin time

19.2 sec

Activated partial thromboplastin time

29.7 sec

Thrombin time

17.5 sec

Fibrinogen

2.05 g/L

5.Liver Fibrotest

Hyaluronic acid

143.26 ng/ml

Laminin

31.52 ng/ml

Collagen Ⅳ

22.96 ng/ml

Procollagen Ⅲ

24.38 ng/ml

6.Ascites

Ascites

13 mm

7.Portal Vein Diameter

Portal vein diameter

13.4 mm

8.Neoplastic Lesions

Neoplastic lesions

No

9.Portal Vein Thrombosis

Portal vein thrombosis

No

10.Liver Stiffness

Liver stiffness

29.5 KPA

11.Fat Attenuation Parameters

Fat attenuation parameters

241 db/m

12.Child-Pugh Scores

Child-Pugh scores

7

7.Follow-up 3
1.Clinical Symptoms

Clinical symptoms

腹痛好转

2.Blood Routine Tests

Hemoglobin

140 g/L

Red blood cell

5.4 *10^12/L

Platelet

140 *10^9/L

White blood cell

5.9 *10^9/L

3.Serum Liver Function Tests

Alanine aminotransferase

27 U/L

Aspartate aminotransferase

30 U/L

Total bilirubin

19.48 µmol/L

Albumin

44 g/L

4.Coagulation Function Tests

Prothrombin time

14.1 sec

Activated partial thromboplastin time

32.8 sec

Thrombin time

15.5 sec

Fibrinogen

1.51 g/L

5.Liver Fibrotest

Hyaluronic acid

151.7 ng/ml

Laminin

28.71 ng/ml

Collagen Ⅳ

24.9 ng/ml

Procollagen Ⅲ

27.45 ng/ml

6.Ascites

Ascites

10 mm

7.Portal Vein Diameter

Portal vein diameter

13 mm

8.Neoplastic Lesions

Neoplastic lesions

No

9.Portal Vein Thrombosis

Portal vein thrombosis

No

10.Liver Stiffness

Liver stiffness

19 KPA

11.Fat Attenuation Parameters

Fat attenuation parameters

260 db/m

12.Child-Pugh Scores

Child-Pugh scores

6

8.Follwo-up 4
1.Clinical Symptoms

Clinical symptoms

腹痛好转

2.Blood Routine Tests

Hemoglobin

137 g/L

Red blood cell

5.51 *10^12/L

Platelet

137 *10^9/L

White blood cell

2.7 *10^9/L

3.Serum Liver Function Tests

Alanine aminotransferase

40 U/L

Aspartate aminotransferase

53 U/L

Total bilirubin

51.6 µmol/L

Albumin

41.7 g/L

4.Coagulation Function Tests

Prothrombin time

14.7 sec

Activated partial thromboplastin time

33.7 sec

Thrombin time

16.2 sec

Fibrinogen

1.93 g/L

5.Liver Fibrotest

Hyaluronic acid

96.81 ng/ml

Laminin

26.41 ng/ml

Collagen Ⅳ

75.09 ng/ml

Procollagen Ⅲ

77.38 ng/ml

6.Ascites

Ascites

0 mm

7.Portal Vein Diameter

Portal vein diameter

14 mm

8.Neoplastic Lesions

Neoplastic lesions

No

9.Portal Vein Thrombosis

Portal vein thrombosis

No

10.Liver Stiffness

Liver stiffness

19.2 KPA

11.Fat Attenuation Parameters

Fat attenuation parameters

247 db/m

12.Child-Pugh Scores

Child-Pugh scores

7

9.Management system of participants
10.Speciman management system
11.Data management system
12.ADR/ADE management system
13.Instruments management system
14.SOPs
1.Treatment SOP
2.Nursing SOP
15.Research team
1.Institutions
2.Researchers