Age:50-75 years old, >5 years of T2DM history, no insulin injection, take 2-3 hypoglycermic drugs, HbA2c>8% before included, no trauma or inflammation at test regions

Age:50-75 years old, no T2DM history, no trauma or inflammation at test regions

Out of the inclusion criteria

glycated hemoglobin (before test on the 0th day)

9.5 %

Fasting plasma glucose (before test on the 0th day)

275 mg/dL

Fasting plasma glucose (after test on the 0th day)

277 mg/dL

insulin(before test on the 0th day)

7.56 μIU/mL

insulin(after test on the 0th day)

3.79 μIU/mL

glucagon(before test on the 0th day)

62.031 pg/mL

glucagon(after test on the 0th day)

50.020 pg/mL

superoxide dismutase (before test on the 0th day)

47.96 %

superoxide dismutase(after teston the 0th day)

50.81 %

heart rate variability(before test on the 0th day)

11

heart rate variability(the 20th min after test on the 0th day)

9

heart rate variability(the 40th min after test on the 0th day)

12

heart rate variability(the 60th min after test on the 0th day)

13

normalized HF ratio (nHF)(before test on the 0th day)

47 %

normalized HF ratio (nHF)(the 20th min after test on the 0th day)

60 %

normalized HF ratio (nHF)(the 40th min after test on the 0th day)

21 %

normalized HF ratio (nHF)(the 60th min after test on the 0th day)

44 %

normalized LF ratio (nLF)(before test on the 0th day)

53 %

normalized LF ratio (nLF)(the 20th min after test on the 0th day)

40 %

normalized LF ratio (nLF)(the 40th min after test on the 0th day)

79 %

normalized LF ratio (nLF)(the 60th min after test on the 0th day)

56 %

glycated hemoglobin (before test on the 56th day)

10.7 %

Fasting plasma glucose (before test on the 56th day)

273 mg/dL

Fasting plasma glucose (after test on the 56th day)

254 mg/dL

insulin (before test on the 56th day)

5.20 μIU/mL

insulin (after test on the 56th day)

3.80 μIU/mL

glucagon(before test on the 56th day)

38.975 pg/mL

glucagon(after test on the 56th day)

34.386 pg/mL

superoxide dismutase(before test on the 56th day)

42.07 %

superoxide dismutase(after test on the 56th day)

45.52 %

heart rate variability(before test on the 56th day)

11

heart rate variability(the 20th min after test on the 56th day)

9

heart rate variability(the 40th min after test on the 56th day)

14

heart rate variability(the 60th min after test on the 56th day)

13

normalized HF ratio (nHF)(before test on the 56th day)

42 %

normalized HF ratio (nHF)(the 20th min after test on the 56th day)

72 %

normalized HF ratio (nHF)(the 40th min after test on the 56th day)

43 %

normalized HF ratio (nHF)(the 60th min after test on the 56th day)

60 %

normalized LF ratio (nLF)(before test on the 56th day)

57 %

normalized LF ratio (nLF)(the 20th min after test on the 56th day)

28 %

normalized LF ratio (nLF)(the 40th min after test on the 56th day)

57 %

normalized LF ratio (nLF)(the 60th min after test on the 56th day)

40 %